This is the practical-exam skill, and almost no set of notes teaches it as a procedure. Work in this order and stop as soon as the answer is settled.
Step 1 — root or shoot? Look at the outermost layer. A cuticle on the epidermis means stem or leaf. Unicellular root hairs and no cuticle mean root.
Step 2 — how are the vascular bundles arranged? - In a ring → dicot stem - Scattered through the ground tissue → monocot stem - Xylem and phloem on separate radii, alternating → root
Step 3 — which way does the protoxylem point? This is the decisive test and it is the one students are rarely taught to use. - Protoxylem toward the centre, metaxylem outward — endarch — this is a stem - Protoxylem toward the periphery, metaxylem inward — exarch — this is a root
If steps 1 and 2 left any doubt, step 3 removes it.
Step 4 — is there a cambium between xylem and phloem? Present means the bundle is open, so secondary growth is possible — a dicot stem. Absent means closed — a monocot stem.
Step 5 — is the ground tissue differentiated? A dicot stem and a root show distinct cortex, endodermis, pericycle and pith. A monocot stem is largely undifferentiated ground tissue throughout.
Worked case. A section shows a cuticle, bundles arranged in a ring, a cambium strip between xylem and phloem in each bundle, protoxylem facing the centre, and a clear pith.
- Step 1: cuticle → shoot, not root.
- Step 2: ring → dicot stem.
- Step 3: endarch → confirms stem.
- Step 4: cambium present → open bundle, dicot.
- Step 5: pith present and tissue differentiated → consistent.
Conclusion: transverse section of a young dicot stem. Write the reasoning out like that, not just the name. The identification alone is one mark; the justification is the rest.
The commonest error is deciding from the shape of the section or the number of bundles. Neither is reliable — both change with the age of the organ and where along it the cut was made.